2x saline sodium citrate ssc buffer (Thermo Fisher)

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Thermo Fisher 2x saline sodium citrate ssc buffer
2x Saline Sodium Citrate Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2x+saline+sodium+citrate+ssc+buffer/SSC+buffer/pmc13014935-338-6-11
Average 97 stars, based on 1 article reviews

2x saline sodium citrate ssc buffer - by Bioz Stars, 2026-10

97/100 stars

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Saline:

Article Title: PhuS conformational dynamics are essential for DNA binding and heme-responsive control of the prrF operon in Pseudomonas aeruginosa
Article Snippet: The qRT-PCR reactions were carried out on StepOne and QuantStudio3 (Applied Biosystems) qPCR machines using TaqMan FastStart Universal Probe Master Mix (Roche) according to manufacturer’s instructions and gene specific primers ( ). .. The membranes were then washed in 2X saline-sodium citrate (SSC) buffer (Invitrogen) for 5 min at 25 °C and RNA immobilized by UV crosslinking for 2 min. .. The membrane was prehybridized in ULTRAhyb Ultrasensitive Hybridization Buffer (ThermoFisher) for 30 min at 68 ̊C, followed by overnight hybridization at 42 ̊C with 0.1 nM PrrF1 or PrrF2 biotinylated northern blot probes.

Article Title: PhuS conformational dynamics are essential for DNA binding and heme-responsive control of the prrF operon in Pseudomonas aeruginosa.
Article Snippet: The samples were then boiled at 95 °C for 4 min and 25 μL of each mixture was loaded on to a 10% Mini-PROTEAN TBE-Urea Gel (Bio-Rad) that was preran for 1 h at 180 V. The samples were run on the gel for approximately 1.5 h at 120 V, followed by transfer to a piece of BrightStar-Plus positively charged nylon membrane (Invitrogen) at 0.5 Amp for 30 min. .. The membranes were then washed in 2X saline-sodium citrate (SSC) buffer (Invitrogen) for 5 min at 25 oC and RNA immobilized by UV crosslinking for 2 min. .. 1 ng of total RNA was diluted to 14 μL with nuclease-free water and added to 14 μL of 2X RNA Loading Dye (Thermo Scientific).

Article Title: Rapid and precise genome engineering in a naturally short-lived vertebrate
Article Snippet: .. Slides were dehydrated and permeabilized in pre-chilled 100% methanol (Sigma-Aldrich, HPLC grade) with 1% Triton X-100 (Fisher Scientific, BP151) at –20 °C for 15 min followed by washing three times in 2X saline sodium citrate (SSC) buffer with 0.1% Tween-20 (2x SSCT; made from 20x SSC, ThermoFisher, AM9763) at room temperature for 30 min each. .. Slides were equilibrated in hybridization buffer (2x SSCT, 10% (w/v) dextran sulfate [Sigma Aldrich, D6001], 10% (v/v) formamide [Thermo Fisher, AM9342]) for 30 min at 37 °C.

Article Title: The RNA-binding protein RSRC2 promotes mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1
Article Snippet: This was followed by a 30-min wash with 4,6-diamidino-2-phenylindole (DAPI) in wash buffer A to counterstain the nuclei (5 ng/ml; D9542 Sigma) at 37° C in the dark. .. The last wash was done with Stellaris® wash buffer B (SMF-WB1-20, LGC Biosearch Technologies) at RT for 5 min. For signal enhancement, coverslips were equilibrated with Base Glucose Buffer composed of 2x saline-sodium citrate (SSC) buffer (10515203 20x UltraPureTM InvitrogenTM, Fisher Scientific), 0.4% glucose solution (∼20% in H2O BioUltra, 49163, Sigma-Aldrich) and 20 mM RNase-free Tris pH 8.0 (1 M, 10259194, Fisher Scientific) in RNase-free H 2 O. .. Then, coverslips were incubated for 5 min in Base Glucose Buffer supplemented with a 1:100 dilution of glucose oxidase (3.7 mg/ml; G7141, Sigma-Aldrich) and catalase (4 mg/ml; C1345, Sigma-Aldrich).

Article Title: Neph1 is required for neurite branching and is negatively regulated by the PRRXL1 homeodomain factor in the developing spinal cord dorsal horn.
Article Snippet: The frozen sections were air-dried, washed with PBS for 15 min at room temperature (RT), and then treated with 0.5 μg/ml proteinase K (PK; Roche) in PBS for 5 min at RT for better probe tissue penetration. .. PK was inactivated with 1 M glycine (Sigma) for 10 min, after which the tissue sections were postfixed in 4% PFA in PBS for 15 min. Then, the sections were acetylated with acetylation buffer (100 mM triethanolamine-HCl, pH 8 and 0.25% acetic anhydride (Sigma)) for 15 min at RT, prehybridized with prewarmed hybridization buffer (50% formamide (Acros Organics), 2x saline sodium citrate (SSC) buffer, 50 μg/mL yeast RNA (Sigma), 1x Denhardt ́s solution (Sigma) and 1 mg/mL salmon sperm DNA (Sigma)) for 1 h at 70 °C and hybridized with prewarmed hybridization buffer containing 1 μg/mL of a Neph1 digoxigenin (DIG)–labeled RNA probe overnight at 65 °C. .. Afterwards, the sections were first washed with prewarmed washing buffer (20% formamide, 0.8x SSC, and 1% Tween-20) for 2 h at 70 °C and then washed with Tris-buffered saline (TBS) supplemented with 0.1% Tween 20 (TBS-T), followed by incubation with blocking buffer (10% sheep inactivated serum in TBS-T) and incubation with an anti-DIG antibody coupled to alkaline phosphate (1:2000; Roche, 11093274910) in blocking buffer overnight at 4 °C.

Article Title: CRL3 ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription.
Article Snippet: .. Total protein was stained with 1 mMAlexa488-NHS or Alexa647-NHS in 50 mM carbonate buffer at a pH of 9.2 for 15 minutes. e9 Molecular Cell 84, 4808–4823.e1–e13, December 19, 2024 mRNA poly(A) fluorescence in situ hybridisation Assays were performed similarly to previous descriptions.4 All steps were followed by three PBS washes (cell fixation and permeabilisation) or 2X saline sodium citrate (SSC) buffer (FISH steps; Thermo Fisher Invitrogen AM9763). ..

Article Title: Rapid and precise genome engineering in a naturally short-lived vertebrate
Article Snippet: .. Slides were dehydrated and permeabilized in pre- chilled 100% methanol (Sigma- Aldrich, HPLC grade) with 1% Triton X- 100 (Fisher Scientific, BP151) at –20 °C for 15 min followed by washing three times in 2X saline sodium citrate (SSC) buffer with 0.1% Tween- 20 (2x SSCT; made from 20x SSC, ThermoFisher, AM9763) at room temperature for 30 min each. .. Slides were equilibrated in hybridization buffer (2x SSCT, 10% (w/v) dextran sulfate [Sigma Aldrich, D6001], 10% (v/v) formamide [Thermo Fisher, AM9342]) for 30 min at 37 °C.

High Performance Liquid Chromatography:

Article Title: Rapid and precise genome engineering in a naturally short-lived vertebrate
Article Snippet: .. Slides were dehydrated and permeabilized in pre- chilled 100% methanol (Sigma- Aldrich, HPLC grade) with 1% Triton X- 100 (Fisher Scientific, BP151) at –20 °C for 15 min followed by washing three times in 2X saline sodium citrate (SSC) buffer with 0.1% Tween- 20 (2x SSCT; made from 20x SSC, ThermoFisher, AM9763) at room temperature for 30 min each. .. Slides were equilibrated in hybridization buffer (2x SSCT, 10% (w/v) dextran sulfate [Sigma Aldrich, D6001], 10% (v/v) formamide [Thermo Fisher, AM9342]) for 30 min at 37 °C.

Hybridization:

Staining:

Fluorescence:

In Situ:

Fluorescence In Situ Hybridization:

other:

Article Title: Cell type-specific intronic RNAs shape genome architecture during neuronal lineage specification
Article Snippet: We purified the RNA product with RNAClean XP magnetic beads (Beckman Coulter, cat. no. A63987) and converted it to single-stranded DNA (ssDNA) by reverse transcription using the Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific, cat. no. EP0752).

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